Product details

Anewex Plasmid Miniprep Kit isolates up to 20μg of DNA plasmid from 600μl to 3ml of bacterial culture in 15 minutes

• Ready-to-use plasmid DNA in minutes.
• Reproducible yields of molecular biology grade plasmid DNA. 
• Single protocol for high- and low-copy vectors.

Applications

The Anewex Miniprep Kit provides reproducible yields of high-purity DNA suitable for use in most application, including: 

• PCR
• Restriction digestion
• Ligation and transformation
• Sequencing
• Screening

Protocol

1. Pellet 1-5 mL bacterial overnight culture by centrifugation at > 8000 rpm for 3 min at room temperature (15-25^oC)
2. Resuspend pelleted bacterial cells in 200 μL buffer Pm1 and transfer to a microcentrifuge tube.
3. Add 200 μL buffer Pm2 and mix through by inverting the tube 4-6 times until the solution becomes clear.
4. Add 300 μL buffer Pm3 and mix immediately and throughly by inverting the tube 4-6 times. 
5. Centrifuge for 10 min at 13000 rpm.
6. Apply the supernatant from Step 5 to the Anewex spin column by decanting or pipetting.
7. Close the lid gently and centrifuge for 30 s at ≥10,000 rpm. Discard the flow-through.
8. Add 500 μL buffer Pm4 to the spin column.
9. Close the lid gently and centrifuge for 15 s at ≥10,000 rpm. Discard the flow-through.
10. Add 500 μL buffer Pm4 to the spin column.
11. Close the lid gently and centrifuge for 15 s at ≥10,000 rpm. Discard the flow-through.
12. Place the plasmid spin column in a new 1.5 mL collection tube. Add 30-100 μL  DNAse/RNase-free water directly to the center of the spin column membrane.
13. Close the lid gently and centrifuge for 1 min at full speed to elute plasmid. 

Do not elute with less than 10 μL RNase-free water as the spin column membrane will not be sufficiently hydrated.