Anewex RNA cleanup kit


For RNA cleanup and concentration with small elution volumes

50 Anewex RNA cleanup mini spin columns, collection tubes (1.5 mL and 2 mL), RNase-free reagents and buffers




20,020 ¥

Product details

Anewex RNA cleanup kits provide a simple, and quick way to clean up your RNA from pre-purified RNA (by phenol/ chloroform), enzymatic reactions (amplification or labeling reactions), DNAse digestions, etc.

  • Quick procedure delivering high-quality total RNA in minutes
  • Ready-to-use RNA for high performance in any downstream application
  • No phenol/ chloroform extraction, no CsCl gradients, no LiCl or ethanol precipitation
  • Complete removal of RT-PCR inhibitor



RNA isolated with Anewex technology has A260/280 ratios of 1.9-2.1, and A260/230 and are applicable for any downstream assays

  • RNA-seq
  • Quantitative, real-time RT-PCR
  • End-point RT-PCR
  • Northern, dot, and slot blotting
  • Array analysis
  • Poly A + RNA selection



  1. Add 2 volume (recommend: 100 µL) of RCB into 1 volume (recommend: 50 µL) of RNA in 1.5 mL tube.
  2. Mix well, then transfer the sample to RNA cleanup spin column placed in a 2 mL collection tube.
  3. Close the lid gently and centrifuge for 15 s at ≥10,000 rpm. Discard the flow-through
  4. Add 300 µL RCW1 to the spin column.
  5. Close the lid gently and centrifuge for 15 s at ≥10,000 rpm. Discard the flow-through
  6. Add 500 µL RCW2 to the spin column.
  7. Close the lid gently and centrifuge for 15s at ≥10,000 rpm. Discard the flow-through
  8. Place the RNA cleanup spin column in a new 1.5 mL collection tube. Add 15 µL  RNase-free water directly to the center of the spin column membrane.
  9. Close the lid gently and centrifuge for 1 min at full speed to elute RNA. 

Do not elute with less than 10 µL RNase-free water as the spin column membrane will not be sufficiently hydrated.


Supporting data and figures

The efficiency of application such as real time RT-PCR and RNA-Seq depends strongly on the purity of the RNA sample used. To assess RNA purity, the absorbance of RNA at 260 nm and the absorbance of potential contaminants at 280 nm or 230 nm was determined by spectroscopic measurement (e.g. Nanodrop). An A260/A280 ratio of 1.8-2.1 at pH 7.5 is widely accepted as indicative of highly pure RNA. Pure RNA should also yield an A260/A230 ratio of around 2 or slightly higher. After using Anewex RNA cleanup kit for extracted RNA, an A280/A260 and A230/A260 was significantly improved from 1.7 to 1.96 for A280/A260 and from 0.65 to 2.17 for A280/A260.


Fig. 1: Quality of RNA, which was extracted from high fat diet liver tissue using guanidine thiocyanate /phenol/ chloroform, was measured by NanoDrop


Fig. 2: Quality of RNA, which was cleaned up using Anewex RNA cleanup kit, was measured by NanoDrop