Anewex Plasmid Miniprep kit
Anewex Plasmid Miniprep kit
ANX210503 日本語
For purification of up to 20 microgram plasmid DNA.
- Ready-to-use Plasmid DNA in minutes
- Single protocol for high- and low-copy vectors
24,200 ¥
Product details
Anewex Plasmid Miniprep Kit isolates up to 20μg of DNA plasmid from 600μl to 3ml of bacterial culture in 15 minutes
- Ready-to-use plasmid DNA in minutes.
- Reproducible yields of molecular biology grade plasmid DNA.
- Single protocol for high- and low-copy vectors.
Applications
The Anewex Miniprep Kit provides reproducible yields of high-purity DNA suitable for use in most application, including:
- PCR
- Restriction digestion
- Ligation and transformation
- Sequencing
- Screening
Protocol
- Pellet 1-5 mL bacterial overnight culture by centrifugation at > 8000 rpm for 3 min at room temperature (15-25oC)
- Resuspend pelleted bacterial cells in 200 μL buffer Pm1 and transfer to a microcentrifuge tube.
- Add 200 μL buffer Pm2 and mix through by inverting the tube 4-6 times until the solution becomes clear.
- Add 300 μL buffer Pm3 and mix immediately and throughly by inverting the tube 4-6 times.
- Centrifuge for 10 min at 13000 rpm.
- Apply the supernatant from Step 5 to the Anewex spin column by decanting or pipetting.
- Close the lid gently and centrifuge for 30 s at ≥10,000 rpm. Discard the flow-through.
- Add 500 μL buffer Pm4 to the spin column.
- Close the lid gently and centrifuge for 15 s at ≥10,000 rpm. Discard the flow-through.
- Add 500 μL buffer Pm4 to the spin column.
- Close the lid gently and centrifuge for 15 s at ≥10,000 rpm. Discard the flow-through.
- Place the plasmid spin column in a new 1.5 mL collection tube. Add 30-100 μL DNAse/RNase-free water directly to the center of the spin column membrane.
- Close the lid gently and centrifuge for 1 min at full speed to elute plasmid.
Do not elute with less than 10 μL RNase-free water as the spin column membrane will not be sufficiently hydrated.