Correspondence work

  • Design and preparation of gene expression vector.
  • Preparation of transgenic cells and genetically modified cells. 
  • Viral vector preparation
  • Molecular biological analysis (real time PCR, microarray, immunoprecipitation, Western blot, Southern blot, Nothern blot, etc.)
  • Cell biological analysis (immunostaining, in situ hybridization, flow cytometer)
  • Epigenetic analysis (MSP, bisulfite sequence, ChIP-PCR, etc.)

Flow of gene cloning services

01
Consultant

We will listen and recommend the solutions for your problems

  • Vector design (selection of target gene acquisition method, selection of basic vector)
  • Selection of gene transfection methods
  • Selection of expression analysis method for transgene

We are able to support complicated vector construction

  • Simultaneous expression of multiple genes (IRES, T2A sequence, etc.)
  • Construction of inducible expression system (Tet induction system, etc)
  • Changes of the gene for antibiotic selection
  • Changes of promoters
  • Addition of tag sequence, fluorescent protein, etc.
02
Construction

Construction of expression vector

We will perform all the process from PCR amplification, synthetic genes to construction of a vector that expresses your interest gene.

03
Transfection/Transduction

Introduction of expression vector into cells

Introduce the expression vector by one of following methods:

  • Lipofection
  • Electroporation
  • Viral infection
04
Selection

Selection of vector-introduced cells (infected cells)

  • Select vector-introduced cells by proper antibiotics
  • Increase the selected clones
05
Analysis

Expression analysis of transgene in infected cells

We will propose the most suitable methods according to your infected cell using purpose

  • Quantitative RT-PCR
  • Western blotting
  • Flow cytometry
  • Immunocytometry / Immunostaining. 

With Anewex R & D, you can do it!

Preparation viral vector / ready virus particle for infection

  • Adeno-associated virus
  • Adenovirus
  • Lentivirus
  • Retrovirus

Gene editing using CRISPR / Cas9 system

  • Guide RNA design and evaluation of cleavage ability
  • Vector design and construction
  • Establish knockout (knock-in) cells